GeneTex

  • Country / Location Selection

United States (US)

Chikungunya virus nsP3 antibody

Anti-Chikungunya virus nsP3 antibody used in Western Blot (WB). GTX135189
Anti-Chikungunya virus nsP3 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135189
Anti-Chikungunya virus nsP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135189

Cat. No. GTX135189

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Chikungunya Virus
Package
100 μl ($399),
25 μl ($169)
See all Chikungunya virus nsP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chikungunya Virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Chikungunya virus nsP3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909832

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Chikungunya virus nsP3 antibody used in Western Blot (WB). GTX135189

GTX135189 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP3 antibody (GTX135189) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chikungunya virus nsP3 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135189

GTX135189 IHC-P (cell pellet) Image

Chikungunya virus nsP3 antibody detects Chikungunya virus nsP3 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and Chikungunya virus nsP3 transfected 293T cell FFPE Cell Pellet Block.
Green: Chikungunya virus nsP3 stained by Chikungunya virus nsP3 antibody (GTX135189) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Chikungunya virus nsP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135189

GTX135189 ICC/IF Image

Immunofluorescent analysis of mock and Chikungunya virus-infected cells using Chikungunya virus nsP3 antibody (GTX135189).
Sample: Chikungunya virus non-infected and infected cells slide.
Green: Chikungunya virus nsP3 antibody (GTX135189) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Garcia G Jr et al. Cell Rep Med 2023; 4 (5) : 101024 Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses.
Submit a Reference

REVIEW

There are currently no reviews for Chikungunya virus nsP3 antibody (GTX135189). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169